The Prionics antibody 6H4 is the most widely used antibody in prion research. This highly versatile anti-prion antibody is used in many applications and can detect prion proteins in humans, cattle, sheep, rabbits, mink and various primates. To make research easier, Prionics now also offers 6H4 conjugates for one-step detection of prion proteins.

Prion diseases are infectious diseases of the brain caused by altered prion proteins. A definitive diagnosis of prion diseases relies on the detection of disease-specific prion proteins. Prionics offers a versatile antibody (6H4) that recognizes most known mammalian prion proteins. The antibody is applied in several methods that are generally used to diagnose prion diseases including; Western Blot, ELISA, immunohistochemistry (IHC), immunoprecipitation, PET blot and FACS analysis. Its use is described in over 65 scientific publications and 6H4 is also the principal component of all of Prionics^®-Check TSE kits.

About mAb 6H4
Monoclonal antibody (mAb) 6H4 is a mouse IgG1 subtype antibody, light chain: Κ subtype. mAb 6H4 recognizes the sequence DYEDRYYRE of the prion protein (human PrP: amino acids 144 - 152). This sequence is conserved in most known mammalian PrP sequences (human, cattle, sheep, rabbit, mink, and a variety of primates). In mice, hamsters and rats, the tyrosine at position 145 is replaced by a tryptophan. However, mAb 6H4 reacts on Western blots with mouse and hamster PrP, indicating that the substitution at position 145 does not prevent binding of 6H4.

6H4 conjugates
Prionics now offers 6H4 coupled to horseradish peroxidase (6H4-POD) and biotin (6H4-Biotin) conjugates for easy detection of prion proteins. Custom-made conjugates are available upon request. Contact info@prionics.com to check which conjugates we can offer.