In a recent publication of The Veterinary Journal, scientists reported on the test results for the Prionics®-Check PrioSTRIP and its ability to recognize PrPSc in different regions of the brain. The Prionics test produced excellent results that were comparable to those of two other Prionics®-Check tests, the WESTERN and the LIA. This performance makes the PrioSTRIP® an excellent diagnostic tool for the characterization of BSE cases.
The Prionics®-Check PrioSTRIP is a chromatographic immunoassay for the detection of Bovine Spongiform Encephalopathy (BSE) in brain samples. A recent publication of The Veterinary Journal, a study conducted by Vidal et al. reported that the Prionics®-Check PrioSTRIP was used to analyze 16 different areas of the brain, of nine confirmed BSE cases, for the presence of PrPSc. The study showed that the PrioSTRIP® successfully detected PrPSc in all analyzed regions of the brain. A PrPSc distribution curve (BPDC) was obtained from the results by plotting the concentration of PrPSc detected in each of the anatomical regions of the brain against the longitudinal axis of the bovine brain.
The Prionics®-Check PrioSTRIP is a chromatographic immunoassay for the detection of Bovine Spongiform Encephalopathy (BSE) in brain samples. A recent publication of The Veterinary Journal, a study conducted by Vidal et al. reported that the Prionics®-Check PrioSTRIP was used to analyze 16 different areas of the brain, of nine confirmed BSE cases, for the presence of PrPSc. The study showed that the PrioSTRIP® successfully detected PrPSc in all analyzed regions of the brain. A PrPSc distribution curve (BPDC) was obtained from the results by plotting the concentration of PrPSc detected in each of the anatomical regions of the brain against the longitudinal axis of the bovine brain.
PrioSTRIP® excellent tool for characterization of atypical BSE
Comparison of the BPDC of the nine BSE cases studied showed that all cases had a similar PrPBSE distribution in the brain however variable intensities were found. An explanation could be that this represents different stages of disease progression. Interestingly, the distribution curve obtained with the Prionics® Check PrioSTRIP closely aligns with those of the Prionics®-Check WESTERN and LIA. All three tests gave the highest signals in the obex region in the brainstem, which is the recommended region for taking BSE samples.
The authors of the publication propose that the BPDC be a method to differentiate classical cases from atypical cases of BSE and conclude that the PrioSTRIP® is an excellent tool for this characterization of atypical BSE cases.
